Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Luteolin Inhibits the Biofilm Formation and Cytotoxicity of Methicillin-Resistant Staphylococcus aureus via Decreasing Bacterial Toxin Synthesis

doi: 10.1155/2022/4476339

Figure Lengend Snippet: Luteolin decreased the cytotoxicity of MRSA, and downregulated the expression of the hemolysin and hlaA genes in MRSA (a) Human bronchial epithelial cells were infected with luteolin-treated MRSA N315, and then the viability of human bronchial epithelial cells was measured using the CCK-8 assay. (b) Expressions of α -hemolysin and δ -hemolysin in luteolin-treated MRSA N315 were evaluated by western blot. (c) Level of hlaA in luteolin-treated MRSA N315 was detected using RT-PCR, and 16S was used as an endogenous control. ∗∗∗ P < 0.001, vs. Blank; ## P < 0.01, ### P < 0.001 vs. Control. (MRSA: methicillin-resistant Staphylococcus aureus , CCK-8: Cell Counting Kit-8, 16S: 16S rRNA, a -hemolysin: alpha hemolysin, d -hemolysin: delta hemolysin, RT-PCR: Real-Time PCR).

Article Snippet: Human bronchial epithelial cells (HBEs; CL-0346) were purchased from Procell (Wuhan, China) and grown in the specific cell medium (CM-0346, Procell, Wuhan, China).

Techniques: Expressing, Infection, CCK-8 Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control, Cell Counting, Real-time Polymerase Chain Reaction

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA NKILA inhibits proliferation and migration of lung cancer via IL-11/STAT3 signaling

doi:

Figure Lengend Snippet: The expression of NKILA was higher in normal human bronchial epithelial cell line. For (A) and (B), the expression level of lncRNA-NKILA was analyzed by Q-PCR. The expression of NKILA was analyzed in NSCLC tissues (C), Kaplan-Meier analyses of the correlations between lncRNA-NKILA expression level and survival (D). Values are means ± SEM for n = 7-8. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.

Article Snippet: Cell culture A normal human bronchial epithelial cell line (16HBE) and NSCLC adenocarcinoma cell lines (A549, NCI-H1975) were purchased from the Biopike Biological company.

Techniques: Expressing, Control

Journal: Journal of Virology

Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

doi: 10.1128/JVI.01437-21

Figure Lengend Snippet: Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.

Article Snippet: UNCN1T cells (a human bronchial epithelial cell line; Kerafast catalog number ENC011) were cultured in BEGM media (Bronchial Epithelial Cell Growth Medium; Lonza catalog number CC-3170) in FNC (Athena Enzyme Systems catalog number 0407) coated 96-well plates.

Techniques: MTT Assay, Concentration Assay

Journal: Journal of Virology

Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

doi: 10.1128/JVI.01437-21

Figure Lengend Snippet: SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.

Article Snippet: UNCN1T cells (a human bronchial epithelial cell line; Kerafast catalog number ENC011) were cultured in BEGM media (Bronchial Epithelial Cell Growth Medium; Lonza catalog number CC-3170) in FNC (Athena Enzyme Systems catalog number 0407) coated 96-well plates.

Techniques: Infection, Knock-Out, Inhibition

Journal: Journal of Virology

Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

doi: 10.1128/JVI.01437-21

Figure Lengend Snippet: Combinational effect of remdesivir and MU-UNMC-1 treatment against SARS-CoV-2 infected UNCN1T cells at 24 h postinfection. (A) Dose response curve of remdesivir in SARS-CoV-2 infected UNCN1T cells at 24 hpi in the presence of different fixed concentrations of MU-UNMC-1; (B) Dose-response curve of MU-UNMC-1 in SARS-CoV-2 infected UNCN1T cells at 24 hpi in the presence of a different fixed concentration of remdesivir; (C) Dose-response percent inhibition matrix of single and combined treatment of remdesivir and MU-UNMC-1 in SARS-CoV-2 infected UNCN1T cells at 24 hpi. (D) 3-D interaction landscape between remdesivir and MU-UNMC-2 calculated based on Loewe additive model using SynergyFinder v.2 in SARS-CoV-2 infected UNCN1T cells at 24 hpi (Loewe synergy score -30.69; with most synergistic area score of -21.34).

Article Snippet: UNCN1T cells (a human bronchial epithelial cell line; Kerafast catalog number ENC011) were cultured in BEGM media (Bronchial Epithelial Cell Growth Medium; Lonza catalog number CC-3170) in FNC (Athena Enzyme Systems catalog number 0407) coated 96-well plates.

Techniques: Infection, Concentration Assay, Inhibition

Journal: Journal of Virology

Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

doi: 10.1128/JVI.01437-21

Figure Lengend Snippet: Combinational effect of remdesivir and MU-UNMC-2 treatment against SARS-CoV-2 infected UNCN1T cells at 24 h postinfection. (A) Dose-response curve of remdesivir in SARS-CoV-2 infected UNCN1T cells at 24 hpi in the presence of a different fixed concentration of MU-UNMC-2; (B) dose-response curve of MU-UNMC-2 in SARS-CoV-2 infected UNCN1T cells at 24 hpi in the presence of a different fixed concentration of remdesivir; (C) dose-response percent inhibition matrix of single and combined treatment of remdesivir and MU-UNMC-2 in SARS-CoV-2 infected UNCN1T cells at 24 hpi. (D) 3-D interaction landscape between remdesivir and MU-UNMC-2 calculated based on Loewe additive model using SynergyFinder v.2 in SARS-CoV-2 infected UNCN1T cells at 24 hpi (Loewe synergy score 26.63; with most synergistic area score of 37.25).

Article Snippet: UNCN1T cells (a human bronchial epithelial cell line; Kerafast catalog number ENC011) were cultured in BEGM media (Bronchial Epithelial Cell Growth Medium; Lonza catalog number CC-3170) in FNC (Athena Enzyme Systems catalog number 0407) coated 96-well plates.

Techniques: Infection, Concentration Assay, Inhibition